Laboratory of Biochemistry of Nitrogen Fixation and Nitrogen Metabolism

Alexey F. Topunov
Dr.Sci. (Biology)
Head of Laboratory
INBI, build. 1, room 250
Телефон +7 (495) 660-34-30 ext.199
E-Mailaftopunov@yandex.ru

Research directions:

  • Study of the symbiotic nitrogen-fixing system of legume plants
  • Design of effective biopreparations with nodule bacteria
  • Study of conditions regulation in nitrogen fixing legume nodules and functioning of leghemoglobin – symbiotic hemoglobin of legume plants
  • Study of the nitric oxide metabolism and its metabolic products
  • Study of reactive oxygen and nitrogen species and reactive carbonyl compounds on hemoglobin
  • The study of proteins of cereals (including triticale) and products of their processing
  • Biosynthesis of poly-3-hydroxyalkanoates and alginates by bacteria of the Azotobacter type
  • Study of biodegradation of poly-3-hydroxyalkanoates
  • Preparation of medicamentary systems based on polymer microparticles

Key achievements:

  • Development of technique for intraspecific identification of nodule bacteria based on the hin-region study of the bacterial genome (the method is protected by the RF patent).
  • Development of method for isolation and purification of leghemoglobin and metleghemoglobin reductase – the enzyme that supports it in physiologically active reduced state
  • Study of interaction of erythrocyte hemoglobin (Hb) with reactive oxygen and nitrogen species and reactive carbonyl compounds, including their complex action
  • Implementation of the computer expert system for the diagnostics of anemias of various genesis using hematological indicators reflecting the state of blood, erythrocytes and hemoglobin (in collaboration with MEPhI)
  • Development of microbiological technology for biosynthesis and purification of biodegradable polymers for medical purposes – polyoxyalkanoates (PAA)

 

Selected publications

  1. Kuzma M.M., Topunov A.F., Layzell D.B. Effects of temperature on infected cell O2 concentration and adenylate levels in attached soybean nodules.  — Plant Physiology, 1995, v.107, N 4, p. 1209-1216.(DOI: 10.1104/pp.107.4.1209)
  2. Sikorski M.M., Topunov A.F., Strozycki P., Vorgias C.E., Wilson K.S., Legocki A.B. Cloning and expression of plant leghemoglobin cDNA of Lupinus luteus in Escherichia coli and purification of the recombinant protein. — Plant Science, 1995, v.108, N 1, p.109-117 (DOI: 10.1016/0168-9452(95)04125-e)
  3. Bonartsev A.P., Livshits V.A., Makhina T.A., Myshkina V.L., Bonartseva G.A., Iordanskii A.L. Controlled release profiles of dipyridamole from biodegradable microspheres on the base of poly(3-hydroxybutyrate). — Express Polymer Letters, 2007, v.1, N.12, p. 797–803 (DOI: 10.3144/expresspolymlett.2007.110)
  4. Shumaev К.B., Gubkin А.А., Serezhenkov V.А., Lobysheva I.I., Kosmachevskaya O.V., Ruuge E.К., Lankin V.Z., Topunov А.F., Vanin А.F. Interaction of reactive oxygen and nitrogen species with albumin- and hemoglobin-bound dinitrosyl iron complexes. — Nitric Oxide – Biology and Chemistry, 2008, v.18, N 1, p. 37-46 (DOI: 10.1016/j.niox.2007.09.085)
  5. Shumaev K.B., Kosmachevskaya O.V., Timoshin A.A., Vanin A.F., Topunov A.F. Dinitrosyl iron complexes bound with haemoglobin as markers of oxidative stress. — Methods in Enzymology, 2008, v. 436, p. 441-457 (DOI: 10.1016/S0076-6879(08)36025-X)
  6. Bonartsev A.P., Yakovlev S.G., Zharkova I.I., Boskhomdzhiev A.P., Bagrov D.V., Myshkina V.L., Makhina T.K., Kharitonova E.P., Samsonova O.V., Feofanov A.V., Voinova V.V., Zernov A.L., Efremov Y.M., Bonartseva G.A., Shaitan K.V., Kirpichnikov M.P. Cell attachment on poly(3-hydroxybutyrate)-poly (ethylene glycol) copolymer produced by Azotobacter chroococcum 7B. — BMC Biochemistry, 2013, 14; Article Number: 12 (DOI: 10.1186/1471-2091-14-12)
  7. Bonartsev A., Yakovlev S., Boskhomdzhiev A., Zharkova I., Bagrov D., Myshkina, V., Mahina T., Kharitonova E., Samsonova O., Zernov A., Zhuikov V., Efremov Y., Voinova V., Bonartseva G., Shaitan K. The Terpolymer Produced by Azotobacter Chroococcum 7B: Effect of Surface Properties on Cell Attachment. — PLOS ONE, 2013, 8 (2); Article Number: e57200 (DOI: 10.1371/journal.pone.0057200)
  8. Punina N.V., Zotov V.S., Parkhomenko A.L., Parkhomenko T.U., Topunov A.F. Genetic diversity of Bacillus thuringiensis from different geo-ecological regions of Ukraine by analyzing the 16S rRNA and gyrB genes and by AP-PCR and saAFLP. – Acta Naturae, 2013, V. 5. N 1(16). P. 90-100.
  9. Kosmachevskaya O.V., Shumaev К.B., Nasybullina E.I., Topunov A.F. Formation of nitri- and nitrosylhemoglobin in systems modeling the Maillard reaction. — Clinical Chemistry and Laboratory Medicine, 2014, v. 52. N 1., p. 161-168 (DOI: 10.1515/cclm-2012-0792)
  10. Nasybullina E.I., Nikitaev V.G., Pronichev A.N., Blindar V.N., Kosmachevskaya O.V., Topunov A.F. Expert diagnostic system for hemoglobinopathies using the data on blood, erythrocyte, and hemoglobin state. — Bulletin of the Lebedev Physics Institute. 2015. v. 42, N 7, p. 206-208
  11. Punina N.V., Makridakis N.M., Remnev M.A., Topunov A.F. Whole-genome sequencing targets drug-resistant bacterial infections. — Human Genomics, 2015, v. 9: 19 (DOI: 10.1186/s40246-015-0037-z)
  12. Kosmachevskaya O.V., Shumaev K.B., Topunov A.F. Carbonyl stress in bacteria: causes and consequences. — Biochemistry (Moscow). 2015. v. 80, N 13, p. 1655-1671
  13. Bonartsev A.P., Zernov A.L., Yakovlev S.G., Zharkova I.I., Myshkina V.L., Mahina T.K., Bonartseva G.A., Andronova N.V., Smirnova G.B., Borisova J.A., Kalishjan M.S., Shaitan K.V., Treshalina H.M. New poly(3-hydroxybutyrate) microparticles with paclitaxel sustained release for intraperitoneal administration. – Anti-Cancer Agents in Medicinal Chemistry, 2017, V. 17. N 3. P. 434-441. (DOI: 10.2174/1871520615666160504095433)
  14. Bonartsev A.P., Zharkova I.I., Yakovlev S.G., Myshkina V.L., Махина, Voinova V.V., Zernov A.L., Zhuikov V.A., Akoulina E.A., Ivanova E.V., Kuznetsova E.S., Shaitan K.V., Bonartseva G.A. Biosynthesis of poly(3-hydroxybutyrate) copolymers by Azotobacter chroococcum 7B: a precursor feeding strategy. – Preparative Biochemistry and Biotechnology, 2017, V. 47. N 2. P. 173-184. (DOI: 10.1080/10826068.2016.1188317)
  15. Shumaev K.B., Kosmachevskaya O.V., Nasybullina E.I., Gromov S.V., Novikov A.A., Topunov A.F. New dinitrosyl iron complexes bound with physiologically active dipeptide carnosine. –  Journal of Biological Inorganic Chemistry, 2017. V. 22. N 1. P. 153–160. (DOI: 10.1007/s00775-016-1418-z.)