Group of Biochemistry of Adaptation of Microorganisms

Elena G. Salina
Ph.D., Dr. Sci. (Biology)
Head of the Group
INBI, build. 2, room 207 

 

Телефон +7 (495) 954-40-34
E-Mail elenasalina@yandex.ru

Key words
Mycobacterium tuberculosis, Mycobacterium abscessus, mycobacteria, latent tuberculosis, mycobacteriosis, virulence factors, antibacterial compounds, bacterial small regulatory RNAs, transcriptomics

Research areas:

  • Functional role of small regulatory RNAs in bacteria
  • Virulence factors and “host-pathogen” interaction
  • Mycobacterial transcriptomics
  • New antibacterial compounds and elucidation of their mechanism of action
  • Alternative strategies in therapy of mycobacterial infections
  • Establishment of mechanisms of bacterial resistance to antimicrobial agents

Basic research methods:

  • cultivation of mycobacteria with obtaining metabolically inert forms
  • nucleic acids isolation and purification
  • transcriptomic analysis of mycobacteria by RNA-seq
  • determination of the minimum inhibitory concentration in vitro and ex vivo
  • obtaining target gene ‘knock-outs’ by allelic exchange
  • construction of strains with overexpression of target genes
  • quantitative real-time PCR

 

Publications

  1. Pogodin PV, Salina EG, Semenov VV, Raihstat MM, Druzhilovskiy DS, Filimonov DA, Poroikov VV. Ligand-based virtual screening and biological evaluation of inhibitors of Mycobacterium tuberculosis SAR QSAR Environ Res. 2024 Jan;35(1):53-69.
  2. L. Mulyukin, D.Recchia, N.A. Kostrikina, M.V. Artyukhina, B.A.Martini, A.Stamilla, G.Degiacomi, E.G.Salina et al. Distinct Effects of Moxifloxacin and Bedaquiline on Growing and ‘Non-Culturable’ Mycobacterium abscessus. Microorganisms. 2023; 11(11): 2690.
  3. Martini B.A., Grigorov A.S., Skvortsova Y.V., Bychenko O.S., Salina E.G., Azhikina T.L. Small RNA MTS1338 Configures a Stress Resistance Signature in Mycobacterium tuberculosis. J. Mol. Sci. 2023, 24, 7928
  4. Salina EG, Postiglione U, Chiarelli LR, Recchia D, Záhorszká M, Lepioshkin A, Monakhova N, Pál A, Porta A, Zanoni G, Korduláková J, Kazakova E, Sassera D, Pasca MR, Makarov V, Degiacomi G. A new benzothiazolthiazolidine derivative, 11726172, is active in vitro, in vivo, and against nonreplicating cells of Mycobacterium tuberculosis. 2022. mSphere. e0036922.
  5. Koumpoura CL, Nguyen M, Bijani C, Vendier L, Salina EG, Buroni S, Degiacomi G, Cojean S, Loiseau PM, Benoit-Vical F, García-Sosa AT, Anne Robert, Baltas M. Design of Anti-infectious Agents from Lawsone in a Three-Component Reaction with Aldehydes and Isocyanides. 2022. ACS Omega. 7(40):35635-35655.
  6. Salina EG, Makarov V. Mycobacterium tuberculosis Dormancy: How to Fight a Hidden Danger. 2022. Microorganisms. 10 (12). P. 2334.
  7. Egorova A, Salina EG, Makarov V. Targeting Non-Replicating Mycobacterium tuberculosis and Latent Infection: Alternatives and Perspectives. Int J Mol Sci. 2021 22(24):13317.
  8. Grigorov A, Bychenko O, Salina EG, Skvortsova Y, Mazurova A, Skvortsov T, Kaprelyants A, Azhikina T. Small RNA F6 Provides Mycobacterium smegmatis Entry into Dormancy. Int J Mol Sci. 2021 22(21):11536.
  9. Bychenko O., Skvortsova Y., Ziganshin R., Grigorov A., Aseev L., Ostrik A., Kaprelyants A., Salina E.G., Azhikina T. Mycobacterium tuberculosis Small RNA MTS1338 Confers Pathogenic Properties to Non-pathogenic Mycobacterium smegmatis. Microorganisms 2021, 9, 414.
  10. Monakhova N, Korduláková J, Vocat A, Egorova A, Lepioshkin A, Salina EG, Nosek J, Repková E, Zemanová J, Jurdáková H, Górová R, Roh J, Degiacomi G, Sammartino JC, Pasca MR, Cole ST, Mikušová K, Makarov V. Design and Synthesis of Pyrano[3,2-b]indolones Showing Antimycobacterial Activity. ACS Infect Dis. 2021; 7(1):88-100.
  11. Makarov V, Salina E, Reynolds RC, Kyaw Zin PP, Ekins S. Molecule Property Analyses of Active Compounds for Mycobacterium tuberculosis. J Med Chem. 2020, 63, 17, 8917–8955
  12. Chiarelli LR., Salina EG., Mori G, Azhikina T, Riabova O, Lepioshkin A, Grigorov A, Forbak M, Madacki J, Orena BS, Manfredi M, Gosetti F, Buzzi A, Degiacomi G, Sammartino JC, Marengo E, Korduláková J, Riccardi G, Mikušová K, Makarov V, Pasca MR. New insights into the mechanism of action of the thienopyrimidine antitubercular prodrug TP053. ACS Infect dis 2020; 6(2):313-323.
  13. Skvortsova YV, Salina EG, Burakova EA, Bychenko OS, Stetsenko DA, Azhikina TL. A new Antisense Phosphoryl Guanidine Oligo-2′-O-Methylribonucleotide Penetrates Into Intracellular Mycobacteria and Suppresses Target Gene Expression. Front Pharmacol. 2019, 10:1049.
  14. Salina EG, Grigorov A, Skvortsova Y, Majorov K, Bychenko O, Ostrik A, Logunova N, Ignatov D, Kaprelyants A, Apt A, Azhikina T. MTS1338, A Small Mycobacterium tuberculosisRNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages. Front Cell Infect Microbiol. 2019, 9:405.
  15. Salina EG, Grigorov AS, Bychenko OS, Skvortsova YV, Mamedov IZ, Azhikina TL, Kaprelyants AS. Resuscitation of dormant “non-culturable” Mycobacterium tuberculosisis characterized by immediate transcriptional burst. Front Cell Infect Microbiol 2019, 9:272.
  16. Salina EG, Ekins S. Makarov V. A Rapid method for estimation of the efficacy of potential antimicrobials in humans and animals by agar diffusion assay. Chemical Biology & Drug Design 2019, 93(6):1021-1025.
  17. Salina EG, Huszár S, Zemanová J, Keruchenko J, Riabova O, Kazakova E, Grigorov A, Azhikina T, Kaprelyants A, Mikušová K, Makarov V. Copper-related toxicity in replicating and dormant Mycobacterium tuberculosis caused by 1-hydroxy-5-R-pyridine-2(1H)-thiones. Metallomics 2018, 10(7):992-1002.
  18. Kaprelyants A., Salina EG, Makarov V. How to kill dormant Mycobacterium tuberculosis. Int J Mycobacteriol 2018 7:399-400. doi: 10.4103/ijmy.ijmy_106_18