Group of Biochemistry of Adaptation of Microorganisms

Elena G. Salina
Ph.D., Dr. Sci. (Biology)
Head of the Group
INBI, build. 2, room 207 

Телефон +7 (495) 954-40-47
E-Mail elenasalina@yandex.ru

Key words
Mycobacterium tuberculosis, Mycobacterium abscessus, mycobacteria, latent tuberculosis, antibacterial compounds, “рost-pathogen” interaction, bacterial small regulatory RNAs, transcriptomics

Research areas:

  • Functional role of small regulatory RNAs in bacteria
  • “Host-pathogen” interaction
  • Mycobacterial transcriptomics (in collaboration with Institute of Bioorganic chemistry RAS)
  • Studying new antibacterial compounds and elucidation of their mechanism of action
  • Establishment of mechanisms of bacterial resistance to antimicrobial agents

Basic research methods:

  • Cultivation of mycobacteria with obtaining metabolically inert forms
  • RNA isolation and purification
  • Transcriptomic analysis of mycobacteria by RNA-seq
  • Determination of the minimum inhibitory concentration in vitro (REMA assay) and ex vivo
  • Macrophage infection
  • Obtaining target gene ‘knock-outs’ by allelic exchange
  • Construction of strains with overexpression of target genes
  • Quantitative real-time PCR

 

Selected publications

  1. Egorova A, Salina EG, Makarov V. Targeting Non-Replicating Mycobacterium tuberculosis and Latent Infection: Alternatives and Perspectives. Int J Mol Sci. 2021 Dec 10;22(24):13317. doi: 10.3390/ijms222413317.PMID: 34948114.
  2. Grigorov A, Bychenko O, Salina EG, Skvortsova Y, Mazurova A, Skvortsov T, Kaprelyants A, Azhikina T. Small RNA F6 Provides Mycobacterium smegmatis Entry into Dormancy. Int J Mol Sci. 2021 Oct 26;22(21):11536.
  3. Bychenko O., Skvortsova Y., Ziganshin R., Grigorov A., Aseev L., Ostrik A., Kaprelyants A., Salina E.G., Azhikina T. Mycobacterium tuberculosis Small RNA MTS1338 Confers Pathogenic Properties to Non-pathogenic Mycobacterium smegmatis. Microorganisms 2021, 9, 414.
  4. Monakhova N, Korduláková J, Vocat A, Egorova A, Lepioshkin A, Salina EG, Nosek J, Repková E, Zemanová J, Jurdáková H, Górová R, Roh J, Degiacomi G, Sammartino JC, Pasca MR, Cole ST, Mikušová K, Makarov V.Design and Synthesis of Pyrano[3,2-b]indolones Showing Antimycobacterial Activity. ACS Infect Dis. 2021; 7(1):88-100.
  5. Makarov V, Salina E, Reynolds RC, Kyaw Zin PP, Ekins S. Molecule Property Analyses of Active Compounds for Mycobacterium tuberculosis. J Med Chem. 2020, 63, 17, 8917–8955.
  6. Chiarelli LR., Salina EG., Mori G, Azhikina T, Riabova O, Lepioshkin A, Grigorov A, Forbak M, Madacki J, Orena BS, Manfredi M, Gosetti F, Buzzi A, Degiacomi G, Sammartino JC, Marengo E, Korduláková J, Riccardi G, Mikušová K, Makarov V, Pasca MR. New insights into the mechanism of action of the thienopyrimidine antitubercular prodrug TP053. ACS Infect dis 2020; 6(2):313-323.
  7. Skvortsova YV, Salina EG, Burakova EA, Bychenko OS, Stetsenko DA, Azhikina TL. A new Antisense Phosphoryl Guanidine Oligo-2′-O-Methylribonucleotide Penetrates Into Intracellular Mycobacteria and Suppresses Target Gene Expression. Front Pharmacol. 2019, 10:1049.
  8. Salina EG, Grigorov A, Skvortsova Y, Majorov K, Bychenko O, Ostrik A, Logunova N, Ignatov D, Kaprelyants A, Apt A, Azhikina T. MTS1338, A Small Mycobacterium tuberculosis RNA, Regulates Transcriptional Shifts Consistent With Bacterial Adaptation for Entering Into Dormancy and Survival Within Host Macrophages. Front Cell Infect Microbiol. 2019, 9:405.
  9. Salina EG, Grigorov AS, Bychenko OS, Skvortsova YV, Mamedov IZ, Azhikina TL, Kaprelyants AS. Resuscitation of dormant «non-culturable» Mycobacterium tuberculosis is characterized by immediate transcriptional burst. Front Cell Infect Microbiol 2019, 9:272.
  10. Salina EG, Ekins S. Makarov V. A Rapid method for estimation of the efficacy of potential antimicrobials in humans and animals by agar diffusion assay. Chemical Biology & Drug Design 2019, 93(6):1021-1025.
  11. Salina EG, Huszár S, Zemanová J, Keruchenko J, Riabova O, Kazakova E, Grigorov A, Azhikina T, Kaprelyants A, Mikušová K, Makarov V. Copper-related toxicity in replicating and dormant Mycobacterium tuberculosiscaused by 1-hydroxy-5-R-pyridine-2(1H)-thiones. Metallomics 2018, 10(7):992-1002.
  12. Kaprelyants A., Salina EG, Makarov V. How to kill dormant Mycobacterium tuberculosis. Int J Mycobacteriol2018 7:399-400. doi: 10.4103/ijmy.ijmy_106_18.
  13. Salina EG, Ryabova O, Vocat A, Nikonenko B, Cole ST, Makarov V. New 1-hydroxy-2-thiopyridine derivatives active against both replicating and dormant Mycobacterium tuberculosis. J Infect Chemother 2017, 23(11):794-797.