Laboratory of Microbiology of Anthropogenic habitats


Yuriy V. Litti
Head of Laboratory
INMI, room 204


Телефон +7 (499) 135-04-20

Keywords: Microorganisms and microbial communities of anthropogenic habitats, formation and oxidation of methane, methanogenic archaea, synthrophic bacteria, anaerobic ammonium oxidation (anammox), anammox bacteria, wastewater treatment, anaerobic systems for treatment of solid and semi-solid wastes, landfills, composting, waste water treatment

Laboratory was founded in 1996 under the guidance of Dr. Sci  A.N. Nozhevnikova (formerly – research group of studying of anaerobic degradation of organic wastes in laboratory of lithotrophic microorganisms INMI RAS headed by A.M. RAS G. A. Zavarzin). The main fields of investigation are connected with anaerobic communities and anaerobic and aerobic microorganisms of anthropogenic habitats such as municipal wastewater treatment facilities, industrial and agricultural wates, landfills, sediments of polluted  waterpols. In cooperation with “ECOS” Company, a new biotechnology of wastewater treatment with immobilization of the active sludge and effective nitrogen elimination using anammox bacteria, was developed and implemented.

The head of laboratory, Dr. A. N. Nozhevnikova, and the members of laboratory, V. K. Nekrasova and PHD Yu. V. Litty  have been rewarded  by the Award of the Government of the Russian Federation in the field of science and technology in 2014.

Major fields of investigation:

  • Microorganisms and microbial communities of anthropogenic habitats, including bioreactors, landfills, sediments of contaminated waters.
  • Isolation of anaerobic mesophilic, psychrophilic and thermophilic bacteria and archaea from anthropogenic habitats.
  • Syntrophic microbial associations in methanogenic communities.
  • Microbial processes in composting.
  • Anammox process and anammox bacteria: enrichment, isolation and identification of anammox bacteria and their microbial satellites.
  • Interactions between nitrifying, denitrifying and anammox bacteria in microbial communities of the bioreactors.
  • Microbial aggregation and formation of biofilms and flocs in anammox bioreactors and methane-producing bioreactors.
  • Development of the novel methods and approaches in treatment of landfills and agricultural waste.
  • Development of the novel methods and approaches in wastewater treatment.


Selected publications:

  1. Kotsyurbenko O.R., Nozhevnikova A.N., Soloviova T.I. and Zavarzin G.A. (1996). Methanogenesis at low temperatures by microflora of tundra wetland soil. Antonie van Levenhoek, 69, N1, 75-86
  2. Lettinga G. Rebac S., Parshina S., Nozhevnikova A., van Lier J.B., Stams A.J.M. High-rate anaerobic treatment of wastewater at low temperatures. Appl. Environ. Microbiol., 1999, 65, No. 4, 1696-1702
  3. Simankova M.V., Kotsyurbenko O.R., Stackebrandt E., Kostrikina N.A., Lysenko A.M., Osipov G.A.,  Nozhevnikova A.N. Acetobacterium tundrae sp. nov., a new species of psychrophilic acetogenic bacterium from tundra soil. Arch. Microbiol., 2000, v.174, p.440-447
  4. Kotsyurbenko O.R., Glagolev M.V., Nozhevnikova, A. N., Conrad R.  Competition between homoacetogenic bacteria and methanogenic archaea for hydrogen at low temperature. FEMS Microbiol Еcol., 2001, v. 38, p. 153-159.
  5. Parshina S.N., Kleerebezem R., Sans J.L., Lettinga G., Nozhevnikova A.N., Kostrikina N.A., Lysenko A.M., Stams A.J.M. Soengenia saccharolytica gen. nov., sp. nov. and Clostridium amygdalum sp. nov. two novel anaerobic benzaldehyde-converting bacteria. International Journal of Systematic and Evolutionary Microbiology, 2003, 53 (6), 1791 – 1799
  6. Kotsyurbenko  O.R.,  Chin K.-J.,  Glagolev M..V., Stubner S., Simankova M.V.,  Nozhevnikova A.N., Conrad R. Acetoclastic and hydrogenotrophic methane production and methanogenic populations in an acidic West-Siberian pet bog. Environmental Microbiology, 2004, v.6, p.1159-1173.
  7. Kotsyurbenko O.R., Friedrich M.W., Simankova M.V., Nozhevnikova A.N., Golyshin P.N., Timmis K.N., Conrad R. Shift from acetoclastic to H2-dependent methanogenesis in a West Siberian peat bog at low pH values and isolation of an acidophilic Methanobacterium strain. Appl. Environ. Microbiol. 2007, v.73, p.2344-2348
  8. KallistovaA.Yu., Goel G., Nozhevnikova A.N. Microbial diversity of methanogenic communities in the systems for anaerobic treatment of organic waste // Microbiology. 2014. V. 83. No.5. pp. 462–483.
  9. Botchkova E.A., Plakunov V.K., Nozhevnikova A.N. Dynamics of biofilm formation on microscopic slides submerged in an anammox bioreactor // Microbiology, Vol. 84, No. 3, 2015, p. 456-460.
  10. A. N. Nozhevnikova, E. A. Botchkova , V. K. Plakunov.  Multi-species biofilms in ecology, medicine, and biotechnology. // Microbiology. November 2015, Volume 84,  Issue 6, pp 731-750
  11. Litti, Y. V., Potekhina, M. A., Zhuravleva, E. A., Vishnyakova, A. V., Gruzdev, D. S., Kovalev, A. A., … & Parshina, S. N. (2022). Dark fermentative hydrogen production from simple sugars and various wastewaters by a newly isolated Thermoanaerobacterium thermosaccharolyticum SP-H2. International Journal of Hydrogen Energy, 47(58), 24310-24327.
  12. Zhuravleva, E. A., Shekhurdina, S. V., Kotova, I. B., Loiko, N. G., Popova, N. M., Kryukov, E., … & Litti, Y. V. (2022). Effects of various materials used to promote the direct interspecies electron transfer on anaerobic digestion of low-concentration swine manure. Science of The Total Environment, 156073.
  13. Litti, Y. V., Russkova, Y. I., Zhuravleva, E. A., Parshina, S. N., Kovalev, A. A., Kovalev, D. A., & Nozhevnikova, A. N. (2022). Electromethanogenesis: a Promising Biotechnology for the Anaerobic Treatment of Organic Waste. Applied Biochemistry and Microbiology, 58(1), 19-36.
  14. Kovalev, A. A., Kovalev, D. A., Zhuravleva, E. A., Katraeva, I. V., Panchenko, V., Fiore, U., & Litti, Y. V. (2022). Two-stage anaerobic digestion with direct electric stimulation of methanogenesis: The effect of a physical barrier to retain biomass on the surface of a carbon cloth-based biocathode. Renewable Energy, 181, 966-977.
  15. Nozhevnikova, A. N., Russkova, Y. I., Litti, Y. V., Parshina, S. N., Zhuravleva, E. A., & Nikitina, A. A. (2020). Syntrophy and interspecies electron transfer in methanogenic microbial communities. Microbiology, 89(2), 129-147.
  16. Mardanov, A. V., Beletsky, A. V., Ravin, N. V., Botchkova, E. A., Litti, Y. V., & Nozhevnikova, A. N. (2019). Genome of a Novel Bacterium “Candidatus Jettenia ecosi” reconstructed from the metagenome of an anammox bioreactor. Frontiers in microbiology, 10, 2442.
  17. Botchkova, E. A., Litti, Y. V., Novikov, A. A., Grouzdev, D. S., Bochkareva, E. S., Beskorovayny, A. V., … & Nozhevnikova, A. N. (2018). Description of “Candidatus Jettenia ecosi” sp. nov., a new species of anammox bacteria. Microbiology, 87(6), 766-776.