Laboratory of Molecular Imaging


Alexey A. Bogdanov

Professor, Dr. Sci. (Biology)
Head of Laboratory
INBI, build. 3, room 33
bogdanov.alexei@gmail.com

Key words:
molecular imaging, time-resolved fluorescence, magnetic resonance imaging, proteinases, fluorescent proteins, genetically encoded sensors, inflammation, carcinogenesis


History of the lab foundation
:
The Molecular Imaging Laboratory and the research team of the laboratory are formed in accordance with Decree No. 220 of the Government of the Russian Federation of April 9, 2010 to carry out work under grant No. 14.W03.31.0023 of the Russian Ministry of Education and Science “Visualization and engineering of eukaryotic genomes” under the Institutional development of the research sector ”of the state program of the Russian Federation“ Development of science and technologies ”for 2013–2020.


Research directions:
Laboratory of Molecular Imaging was established with the purpose of solving a number of important scientific problems related to the field of medical biotechnology and nanotechnology. The Laboratory was created on the basis of a scientific team from the Federal State Research Center “Fundamental Foundations of Biotechnology “of the Russian Academy of Sciences. Researchers participating in the project have prominent international standings in their respective areas, and are experts in their fields, i.e. non-invasive imaging in living systems, biophysics, biochemistry and engineering of fluorescent proteins, as well as radiology.

We created an interdisciplinary research team in order to obtain breakthrough results within the framework of several interrelated directions of research at the junction of molecular biology, biophysics and medicine. The main goal of the laboratory is to achieve breakthroughs in the field of mapping eukaryotic genome imaging using multimodal / tomographic and multidisciplinary approaches. Scientific breakthroughs are expected as a result of the convergence of efforts to unite researchers who are currently working in parallel areas with a high level of significance and innovation, thereby strengthening the overall scientific potential. The laboratory is actively engaged in research along the following directions:

  1. development of bioengineering approaches for creating fluorescent sensor molecules based on mutants derived from the Cas family of endonucleases;
  2. application of the achievements of the laboratory of the leading scientist in the field of nanotechnologies for the delivery of recombinant molecules and viral constructs for the purpose of regulated expression of CRISPR / Cas9 based sensors in cells and tissues of experimental animals;
  3. the use of picosecond laser technology to study the fluorescence lifetimes of recombinant sensor molecules in the context of targeting genomic DNA;
  4. the creation of a hybrid imaging technology that combines magnetic resonance imaging and fluorescence visualization of lifetimes in time domain.


Selected publications:

  • Anton Yu. Sdobnov, Maxim E. Darvin, Johannes Schleusener, Jürgen Lademann, Valery V. Tuchin. Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents- quantitative analysis using confocal Raman microscopy. J.Biophotonics jbio.201800283R2  (WoS, Q1, IF 3.768,  SJR 1.03)
  • A. Yu. Sdobnov, J. Lademann, M. E. Darvin, and V. V. Tuchin. Sensors for Proteolytic Activity Visualization and Their Application in Animal Models of Human Diseases, Biochemistry-Moscow, Vol.84, Supplement1, S144-S158. WoS, Q4,  I F 1.724,  SJR 0.16)
  • A.A. Bogdanov, I.D. Solovyev, A.P. Savitsky. Sensors for Proteolytic Activity Visualization and Their Application in Animal Models of Human Diseases. Vol.84, Supplement1, S1-S18.
    (WoS, Q4,  I F 1.724,  SJR 0.16)