Alexander I. Yaropolov
Professor, Dr.Sci. (Chemistry)
Head of Laboratory
INBI, build. 2, room 217
Телефон +7 (495) 954-44-77


General description of the Laboratory:
The Laboratory of Chemical Enzymology was established in 1986. The Laboratory was originally focused on the chemical, structural and electrochemical investigations of the redox enzymes and proteins, as their application in various areas of biotechnology. The major problems facing the laboratory are bioelectrocatalysis; study of mechanisms of redox enzymes and free radical reactions; investigation of environmentally friendly technology of «green» organic synthesis. At present time the Laboratory comprises 6 staff researchers.

The laboratory is headed by Prof. A.I. Yaropolov since that time. He has great experience in bioelectrocatalysis, in studies of the mechanism of redox enzymes and free radical reactions, in development of heterogeneous catalysts based on immobilized enzymes, creation of various types of biosensors. He is one of the authors of the discovery called “bioelectrocatalysis” of oxidoreductases. He is the author of 19 patents and more that 160 publications in refereed journals.

Scientific interests, priorities:

  • physico-chemical study of oxidoreductases and their application in biotechnology;
  • investigation of the mechanism of free radical reactions during oxidoreductase function;
  • fine organic synthesis using oxidoreductases;
  • bioelectrochemical studies of oxidoreductases, including direct (mediator-less) electron transfer and bioelectrocatalysis;
  • using laccase-mediator systems for degradation of xenobiotics, synthesis of conducting polymers and polymer/carbon nanomaterials composites for supercapacitor development;
  • enzyme-mediated polymerization and modification of natural physiologically active compounds;
  • enzymatic reactions in deep eutectic solvents;
  • pretreatment of lignocellulose using deep eutectic solvents.


Selected publications:

  1. Khlupova M., Vasil’eva I., Shumakovich G., Zaitseva E., Chertkov V., Shestakova A., Morozova O., Yaropolov A.. Enzymatic Polymerization of Dihydroquercetin (Taxifolin) in Betaine-Based Deep Eutectic Solvent and Product Characterization. Catalysts. 2021. 11(5). Article number: 639.
  2. Vasil’eva I., Shumakovich G., Khlupova M., Vasiliev R., Morozova O., Yaropolov A. Enzymatic synthesis and electrochemical characterization of sodium 1,2-naphthoquinone-4-sulfonate-doped PEDOT/MWCNT composite. RSC Advances. 2020. 10. 33010-33017.
  3. Vasil’eva I.S., Shumakovich G.P., Morozova O.V., Khlupova M.E., Vasiliev R.B., Zaitseva E.A., Yaropolov A.I. Efficiency of a fungal laccase in 3,4-ethylenedioxythiophene polymerization. Chemical Papers. 2018. 72(6) 1499-1505.
  4. M.E. Khlupova, O.V. Morozova, I.S. Vasil’eva, G.P. Shumakovich, N.V. Pashintseva, L.I. Kovalev, S.S. Shishkin, V.A. Chertkov, A.K. Shestakova, A.V. Kisin, A.I. Yaropolov. Laccase-catalyzed heterocoupling of dihydroquercetin and p-aminobenzoic acid: effect of the reaction product on cultured cells. // Biochemistry (Moscow), 2018, V. 83, № 8, P. 992-1001. DOI: 10.1134/S0006297918080102
  5. G.P. Shumakovich, O.V. Morozova, M.E. Khlupova, I.S. Vasil’eva, E.A. Zaitseva, A.I. Yaropolov. Enhanced performance of a flexible supercapacitor due to a combination of pseudocapacitances of both PANI/MWCNT composite electrode and gel polymer redox electrolyte. // RSC Advances.  2017. V. 7. P. 34192-34196.  DOI: 10.1039/c7ra04801g
  6. G.V. Otrokhov, G.P. Shumakovich, M.E. Khlupova, I.S. Vasil’eva, I.B. Kaplan, B.T. Zaitchik, E.A. Zaitseva, O.V. Morozova, A.I. Yaropolov. Biocatalytic approach as alternative to chemical synthesis of polyaniline/carbon nanotube composite with enhanced electrochemical properties. // RSC Advances.  2016. V. 6. P. 60372-60375.  DOI: 10.1039/c6ra12352j
  7. M.E.Khlupova, I.S.Vasil’eva, G.P.Shumakovich, O.V.Morozova, V.A. Chertkov, A.K. Shestakova, A.V. Kisin, A.I. Yaropolov. Enzymatic polymerization of dihydroquercetin using bilirubin oxidase. // Biochemistry (Moscow), 2015, V 80, №2, P. 233-241. DOI: 10.1134/S0006297915020108
  8. O.D. Hendrickson, O.V. Morozova, A.V. Zherdev, A.I. Yaropolov, S.G. Klochkov, S.O. Bachurin, B.B. Dzantiev. Study of distribution and biological effects of fullerene C60 after single and multiple intragastrical administrations to rats. // Fullerenes, Nanotubes and Carbon Nanostructures. 2015. V. 23(7). P. 658-668.  DOI: 10.1080/1536383X.2014.949695
  9. G.P. Shumakovich, G.V. Otrokhov, M.E. Khlupova, I.S. Vasil’eva, E.A. Zaitseva, O.V. Morozova, A.I. Yaropolov. Laccase-catalyzed synthesis of aniline oligomers and their application for the protection of copper against corrosion. // RSC Advances. 2014. V. 4(57), P. 30193-30196. DOI: 10.1039/c4ra04836a
  10. Khlupova M., Vasil’eva I., Shumakovich G., Morozova O., Chertkov V., Shestakova A., Kisin A., Yaropolov A. Laccase-mediated biotransformation of dihydroquercetin (taxifolin). // Journal of Molecular Catalysis B: Enzymatic. 2016. V. 123. № 1. P. 62-66.
  11. Reshetilov A.N., Kitova A.E., Kolesov V.V., Yaropolov A.I. Mediator-free bioelectrocatalytic oxidation of ethanol on an electrode from thermally expanded graphite modified by gluconobacter oxydans membrane fractions // Electroanalysis.  2015. V. 27 (6).   P. 1443-1448.
  12. G. Otrokhov, D. Pankratov, G. Shumakovich, M. Khlupova,Y. Zeifman, I. Vasil’eva, O. Morozova, A. Yaropolov. «Enzymatic synthesis of polyaniline/multi-walled carbon nanotubecomposite with core shell structure and its electrochemical characterization for supercapacitor application». // Electrochimica Acta. 2014. V. 123. Р. 151-157.
  13. Otrokhov, G.V.; Morozova, O.V.; Vasil’eva, I. S.; Shumakovich, G.P.; Zaitseva, E.A.; Khlupova, M. E.; Yaropolov, A. I. Biocatalytic synthesis of conducting polymers and prospects for its application. // Biochemistry-Moscow. 2013, V.78 (13), P.1539-1553.
  14. Dmitry V. Pankratov, Yulia S. Zeifman, Olga V. Morozova, Galina P. Shumakovich, Irina S. Vasil’eva, Sergey Shleev, Vladimir O. Popov, Alexander I. Yaropolov. «A comparative study of biocathodes based on multiwall carbon nanotube buckypapers modified with three different multicopper oxidases». // Electroanalysis, 2013, V. 25, № 5, 1143-1149.
  15. Shumakovich G., Kurova V, Vasileva I., Pankratov D., Otrokhov G., Morozova O., Yaropolov A. «Laccase-mediated synthesis of conducting polyaniline». // Journal of Molecular Catalysis. B. Enzymology, 2012, V. 77, P. 105-110.
  16. Shumakovich G., Streltsov A., Gorshina E., Rusinova T., Kurova V., Vasil’eva I., Otrokhov G., Morozova O., Yaropolov A. Laccase-catalyzed oxidative polymerization of aniline dimer (N-phenyl-1,4-phenylenediamine) in aqueous micellar solution of sodium dodecylbenzenesulfonate. Journal of Molecular Catalysis B-Enzymatic, 2011, V. 69, № 3-4, P. 83-88.
  17. Shleev S., Shumakovich G., Morozova O., Yaropolov A. Stable ‘floating’ air diffusion biocathode based on direct electron transfer reactions between carbon particles and high redox potential laccase. Fuel Cells, 2010, V. 10, № 4, P. 726-733.
  18. Streltsov A.V., Morozova O.V., Arkharova N.A., Klechkovskaya V.V., Staroverova I.N. Shumakovich G.P., Yaropolov A.I. Synthesis and characterization of conducting polyaniline prepared by laccase-catalyzed method in sodium dodecylbenzenesulfonate micellar solutions. // Journal of Applied Polymer Science. 2009, V. 114, № 2, P. 928-934.
  19. Gorbacheva M., Morozova O., Shumakovich G., Streltsov A.,  Shleev S., Yaropolov A. Enzymatic oxidation of manganese ions catalysed by laccase. // Bioorganic Chemistry.  2009, V. 37, № 1-3, P. 1-5.
  20. Morozova O.V., Shumakovich G.P., Shleev S.V., Yaropolov Ya.I. Laccase-mediator systems and their applications: A review. Applied Biochemistry and Microbiology. 2007, V. 43, № 5, P. 523-535.
  21. I.S. Vasil’eva, O.V. Morozova, G.P. Shumakovich, S.V. Shleev, I.Yu. Sakharov, A.I. Yaropolov «Laccase-catalyzed synthesis of optically active polyaniline». // Synthetic Metals. 2007, V. 157, № 18-20, P. 684-689.
  22. Morozova О.V., Shumakovich G.P., Gorbacheva M.A., Shleev S.V., Yaropolov A.I. “Blue” laccases. Biochemistry-Moscow. 2007, V. 72, № 10, P. 1136-1150.
  23. G.P.Shumakovich, S.V.Shleev, O.V.Morozova, P.S.Khohlov, I.G.Gazaryan, A.I.Yaropolov. Electrochemistry and kinetics of fungal laccase mediators. // Bioelectrochemistry. 2006, V.69, № 1, Р. 16-24.
  24. S. Shleev, P. Persson, G. Shumakovich, Y. Mazhugo, A. Yaropolov, T. Ruzgas, L. Gorton. Interaction of fungal laccases and laccase-mediator systems with lignin. Enzyme and Microbial Technology, 2006, V. 39, № 4, P. 841-847.
  25. S. Shleev, J. Tkac, A. Christenson, T. Ruzgas, A.I. Yaropolov, J.W. Whittaker, L. Gorton. Direct electron transfer between copper-containing proteins and electrodes. // Biosensors & Bioelectronics. 2005, V. 20, № 12, P. 2517-2554.
  26. Shleev S.V., Morozova O.V., Nikitina O.V., Gorshina E.S., Rusinova T.V., Serezhenkov V.A., Burbaev D.S., Gazaryan I.G., Yaropolov A.I. Comparison of physico-chemical characteristics of four laccases from different basidiomycetes. // Biochimie. 2004, V. 86, № 9-10, Р. 693-703.
  27. Karamyshev A.V., Shleev S.V., Koroleva O.V., Yaropolov A.I., Sakharov I.Yu. Laccase-catalyzed synthesis of conducting polyaniline. // Enzyme and Microbial Technology. 2003, V. 33, № 5, P. 556-564.
  28. Yaropolov A.I., Kharybin A.N., Emneus J., Marko-Varga G., Gorton L. Electrochemical properties of some copper-containing oxidases. // Bioelectrochemistry & Bioengenrging. 1996, V. 40, №1, P. 49-57.
  29. Varfolomeev S.D., Kurochkin I.N., Yaropolov A.I. Direct electron transfer effect biosensors. Biosensors & Bioelectronics. 1996, V. 11, № 9, P. 863-871.
  30. Yaropolov A.I., Kharybin A.N., Emneus J., Marko-Varga G., Gorton L. Flow-injection analysis of phenols at graphite electrode modified with co-immobilised laccase and tyrosinase. Anal. Chim. Acta, 1995, v. 308. pp. 137-144.
  31. Yaropolov A.I., Skorobogatko O.V., Vartanov S.S, Varfolomeyev S.D. Laccase – properties, catalytic mechanism, and applicability. // Applied Biochemistry and Biotechnology. 1994, V. 49, № 3, P. 257-280.
  32. Saenko E.L., Yaropolov A.I., Harris E.D. Biological functions of ceruloplasmin expressed through copper-binding sites and a cellular receptor. // Journal of Trace Elements in Experimental Medicine. 1994, V. 7, № 2, P. 69-88.
  33. Pavlov A.R., Revina A.A., Dupin A., Boldyrev A., Yaropolov, A.I. The Mechanism of interaction of carnosine with superoxide radicals in water solutions. // . Biochimica et Biophysica Acta (BBA) – General Subjects.  1993, V. 1157, № 3, P. 304-312.
  34. Yaropolov A., Karyakin A., Varfolomeev S., Berezin I. Mechanism of H2-electrooxidation with immobilized hydrogenase. // Bioelectrochemistry and Bioenergetics, 1984, V. 12, № 3-4, P. 267 – 277.
  35. M.R. Tarasevich, A.I. Yaropolov, V.A. Bogdanovskaya, S.D. Varfolomeev. Electrocatalysis of a cathodic oxygen reduction by laccase. // Bioelectrochemistry and Bioenergetics. 1979, V. 6, № 3, P. 393-403.
  36. Yaropolov A., Tarasevich M., Varfolomeev S. Electrochemical properties of peroxidase. // Bioelectrochemistry and Bioenergetics. 1978, V. 5, № 1, P. 18-24.