Group of Experimental Mycology

Терешина для сайта[1] Vera M. Tereshina
Dr.Sci. (Biology)
Head of the group
INMI, room 306
Телефон +7 (499) 135-01-69
E-Mail v.m.tereshina@inbox.ru

Main research directions:

  • Biochemical adaptation of filamentous fungi to various stress influences
  • Study of resting cells in fungal life cycle
  • Diversity of the lipid composition of eukaryotic cells of microorganisms – representatives of different taxa of lower fungi and Oomycetes
  • Physiological characteristics of lipid metabolism of lower fungi and Oomycetes
  • Study of heterothallism and dimorphism in Mucorales
  • Development of biotechnological foundations for the synthesis of biologically active lipids, using producers of pharmacologically active essential PUFAs
  • Development of new medications, based on fungal biologically active substances

Since 1996, the main objective of the research group was to study the biochemical mechanisms that allow fungi to adapt to various stress impacts. These studies were part of the main research direction, dedicated to biochemical adaptation of organisms to stress. In the laboratory, not only biomembrane lipids were studied, but also other biomolecules involved in biochemical adaptation of fungi – the so-called chemical chaperones – fungal cytosol carbohydrates, i.e. non-cyclic polyols and disaccharides. Among the latter, special attention was given to trehalose, that not only stabilizes the membranes and protects them from free radicals, but also protects cells from heat shock death, by interacting with HSPs. The main stress factors, the effects of which on adaptation were studied, were light, temperature and nutrient-deprivation (starvation). The study of the heat shock effects allowed to suggest 2 new hypothesis about the origins of thermophilic fungi and about protection of the membranes by membrane stabilizing compounds (trehalose, glycolipids, sterols, etc.).

Using three filamentous fungi as an example, the fundamental difference between the response to the heat shock and to the moderate thermal impacts was revealed for the first time. Only in response to heat shock the increase of the level of trehalose and phosphatidic acids was observed, while at the same time the unsaturation degree of phospholipids didn’t decrease. A new hypothesis of stabilization of membrane under heat shock conditions by thermoprotectors was suggested.

The study of heterothallism in Zygomycetes have showed a number of physiological and biochemical features of the (+) and (-) strains. Cooperative synthesis of sexual hormones (trisporic acids) leads to carotenogenesis stimulation and changes in fungal lipogenesis. Study of the composition of trisporoids during stimulation of carotenogenesis by azines allowed to suggest the scheme for increasing their production.

Using Mucor spp. as model organisms, it was found that the age of sporangiospores affects the dimorphism phenomenon – the ability to germinate in both filamentous and yeast types. The use of itraconazole (inhibitor of the ergosterol biosynthesis) allowed to demonstrate dimorphic shift and hyphal growth pattern in the presence of lipids required for polarized growth – ergosterol and triacylglycerols. It is shown that spore germination correlates with the lipid composition and the ratio of their individual classes – TAG, PA, PC/PE and ESt/SSt. The obtained results indicate the role of lipids in the formation of the cell wall and alternative morphotypes of the mucorous fungi.


Selected publications:

  1. Tereshina V. M Thermotolerance in Fungi: The Role of Heat Shock Proteins and Trehalose. Microbiology. 2005. Vol. 74. No. 3. P. 293-304.
  2. Tereshina V. M., Memorskaya, A. S., Kotlova, E. R. & Feofilova, E. P. Membrane lipid and cytosol carbohydrate composition in Aspergillus niger under heat shock // Microbiology. 2010, Vol. 79, P. 40–46 DOI 10.1134/S0026261710010066.
  3. Tereshina V. M., Memorskaya, A. S. & Kotlova, E. R. The effect of different heat influences on composition of membrane lipids and cytosol carbohydrates in mycelial fungi // Microbiology. 2011, Vol. 80, P. 455–460. DOI 10.1134/S0026261711040199.
  4. Vereshchagina O. A., Memorskaya A.S., Tereshina V.M. Trisporoids under the Stimulation of Carotenogenesis in Blakeslea trispora // Microbiology. 2012. Vol. 81, No. 5. P. 526-533. DOI 10.1134/S0026261712050177.
  5. Smolyanyuk E. V., Bilanenko E.N., Tereshina V.M., Kachalkin a. V., Kamzolkina O. V. Effect of sodium chloride concentration in the medium on the composition of the membrane lipids and carbohydrates in the cytosol of the fungus Fusarium sp. // Microbiology (Moscow). 2013. V. 82. № 5. P. 600–608.
  6. Vereshchagina O. A., Tereshina V. M. Trisporoids and Carotenogenesis in Blakeslea trispora // Microbiology. 2014. Vol. 83. No. 5. P. 438–449.
  7. Yanutsevich E. A., Memorskaya A. S., Groza N. V., Kochkina G. A., Tereshina V. M. Heat Shock Response in the Thermophilic Fungus Rhizomucor miehei // Microbiology. 2014. Vol. 83, No. 5. P. 498–504. Doi: 10.1134/S0026261714050282
  8. E.A. Yanutsevich, O.A. Danilova, N.V. Groza, V.M. Tereshina Membrane lipids and cytosol carbohydrates in Aspergillus niger under osmotic, oxidative, and cold impact // Microbiology, 2016, Vol. 85, No 3, 302-310. Doi:10.1134/S0026261716030152
  9. Yanutsevich E. A., Danilova O.A., Groza N. V., Kotlova E. R., Tereshina V. M. Heat shock response of thermophilic fungi: membrane lipids and soluble carbohydrates under elevated temperatures // Microbiology (SGM). 2016. Published Ahead of Print 15.03.2016, doi: 10.1099/mic.0.000279