Extremophiles Metabolism Laboratory

Ilya V. Kublanov
Ph.D. (Biology)
Head of Laboratory
INMI, room 113a
Телефон +7 (499) 135-12-29 ext. 729
E-Mail kublanov.ilya@gmail.com

Laboratory`s main areas of expertise are diversity, physiology and evolution of extremophiles: microorganisms, optimally growing at high temperatures (thermophiles), low pH (acidophiles) or salinity (halophiles), and also polyextremophiles, capable of growing at more than one extreme parameter, e.g. thermoacidophiles etc. In our work we use interdisciplinary approach combining classical microbiology, molecular biology, biochemistry and bioinformatics to perform a complete set of experimental procedures, from collection of environmental samples to obtaining pure cultures and enzymes preparations. Within this field our main interests are:

  1. Isolation and characterization of novel taxa of extremophilc microorganisms.
  2. Microbial ecology of thermal environments (terrestrial, shallow water and deep sea hot vents, deep subsurface biosphere etc.)
  3. Study of uncultivated microorganisms, the so-called microbial dark matter, using both (i) microbiological techniques, based on isolation of pure cultures, followed by their comprehensive characterization, and (ii) cultivation-independent approaches – various meta-omics.
  4. Novel catabolic processes and pathways: novel electron acceptors, donors and donor/acceptor pairs, including insoluble compounds (biopolymers, minerals of polyvalent metals and metalloids, electrodes of bioelectrochemical systems etc.).
  5. Extremophilic microorganisms, capable of hydrolyzing biopolimers: insoluble proteins, polysaccharides.
  6. Extremophilic microorganisms capable of carbon monoxide oxidation.
  7. Ectremophilic microorganisms, producing biogas and biofuels (eg. H2, ethanol, methane).
  8. Extremozymes – enzymes from extremophiles belonging to novel families, involved in novel pathways, promising for biotechnology.

Our laboratory collaborates with many scientific organizations within and beyond Russia. Among others, the following of them may be highlighted:

  • Immanuel Kant Baltic Federal University, Kaliningrad, Russia
  • Lomonosov Moscow State University: the Department of Geochemistry, the Department of General Physics, the Department of Engineering and Ecological Geology, A.N. Belozersky Institute of Physico-Chemical Biology
  • Institute of General and Experimental Biology SB RAS, Ulan-Ude, Russia
  • Gubkin Russian State University of Oil and Gas, Moscow, Russia
  • Lomonosov Northern (Arctic) Federal University, Arkhangelsk, Russia
  • Institute for Marine Coastal Environments, CNR, Italy
  • Bangor University, UK
  • University of Duisburg-Essen, Germany
  • University of the Free State, Bloemfontein, South Africa
  • University of Heidelberg, Germany
  • INRA, Nantes, France
  • University of Lyon, France

 

Selected publications:

  • Aleksei A Korzhenkov, Stepan V Toshchakov, Rafael Bargiela, Huw Gibbard, Manuel Ferrer, Alina V Teplyuk, David L Jones, Ilya V Kublanov, Peter N Golyshin, Olga V Golyshina. Archaea dominate the microbial community in an ecosystem with low-to-moderate temperature and extreme acidity. 2019. Microbiome, volume 7. doi:10.1186/s40168-019-0623-8
  • Camilla L. Nesbø, Rhianna Charchuk, Stephen M. J. Pollo, Karen Budwill, Ilya V. Kublanov, Thomas H. A. Haverkamp, Julia Foght. Genomic analysis of the mesophilic Thermotogae genus Mesotoga reveals phylogeographic structure and genomic determinants of its distinct metabolism. 2019. Environmental Microbiology, Volume 21, pp 456-470. doi:10.1111/1462-2920.14477
  • Dimitry Y. Sorokin, Maria S. Muntyan, Stepan V. Toshchakov, Aleksei Korzhenkov, and Ilya V. Kublanov. Phenotypic and genomic properties of a novel deep-lineage haloalkaliphilic member of the phylum Balneolaeota from soda lakes possessing Na+-translocating proteorhodopsi. Frontiers in microbiolog. 2018. doi:10.3389/fmicb.2018.02672
  • Wohlgemuth R., J. Littlechild, D. Monti, K. Schnorr, T. van Rossum, B. Siebers, P. Menzel, I. V. Kublanov, A. G. Rike, G. Skretas, Z. Szabo, X. Peng, M. J. Young. Discovering novel hydrolases from hot environments. Biotechnology Advances. 2018. doi: 10.1016/j.biotechadv.2018.09.004
  • Kublanov I.V., Sigalova O.M., Gavrilov S.N., Lebedinsky A.V., Rinke C., Kovaleva O., Chernyh N.A., Ivanova N., Daum C., Reddy T.B.K., Klenk H.-P., Spring S., Göker M., Reva O.N., Miroshnichenko M.L., Kyrpides N.C., Woyke T., Gelfand M.S., Bonch-Osmolovskaya E.A. Genomic analysis of Caldithrix abyssi, the thermophilic anaerobic bacterium of the novel bacterial phylum Calditrichaeota. Frontiers Microbiol. 2017. 8:195.
  • Sorokin D, Messina E, Smedile F, Roman P, Sinninghe-Damste J, Golyshin P, Ciordia S, Mena M, Ferrer M, Kublanov I, Samarov N, Toshchakov S., La Cono V and Yakimov M. Discovery of anaerobic lithoheterotrophic haloarchaea, ubiquitous in hypersaline habitats. ISME J. 2017. 11. 1245-1260.
  • Golyshina OV, Toshchakov SV, Makarova KS, Gavrilov SN, Korzhenkov AA, La Cono V, Arcadi E, Nechitaylo TY, Ferrer M, Kublanov IV, Wolf YI, Yakimov MM, Golyshin PN. ‘ARMAN’ archaea depend on association with euryarchaeal host in culture and in situ. Nat Commun. 2017. 8(1): 60.
  • Gavrilov SN, O.A. Podosokorskaya, D.G. Alexeev, A.Y. Merkel, M.A. Khomyakova, M.S. Muntyan, I.A. Altukhov, I.O. Butenko, E.A. Bonch-Osmolovskaya, V. M. Govorun, I.V. Kublanov. Respiratory Pathways Reconstructed by Multi-Omics Analysis in Melioribacter roseus, Residing in a Deep Thermal Aquifer of the West-Siberian Megabasin. Front Microbiol. 2017. 8: 1228.
  • Elcheninov A.G., Menzel P., Gudbergsdottir S.R., Slesarev A.I., Kadnikov V.V., Krogh A., Bonch-Osmolovskaya E.A., Peng X. and Kublanov I.V. Sugar metabolism of the first thermophilic planctomycete Thermogutta terrifontis: comparative genomic and transcriptomic approaches. Front. Microbiol. 2017. 8. 2140. doi: 10.3389/fmicb.2017.02140
  • Gavrilov S. N., Stracke C., Jensen K., Menzel P., Kallnik V., Slesarev A., Sokolova T., Zayulina K., Brasen C., Bonch-Osmolovskaya E. A., Peng X., Kublanov I. V., Siebers B. 2016. Isolation and characterization of the first xylanolytic hyperthermophilic euryarchaeon Thermococcus sp. strain 2319×1 and its unusual multidomain glycosidase. Front Microbiol. 7, 552.
  • Zavarzina D.G., Chistyakova N.I., Shapkin A.V., Savenko A.V., Zhilina T.N., Kevbrin V.V., Alekseeva T.V., Mardanov A.V., Gavrilov S.N., Bychkov A.Yu. 2016. Oxidative biotransformation of biotite and glauconite by alkaliphilic anaerobes: The effect of Fe oxidation on the weathering of phyllosilicates. Chem. Geol. 439:98-109.
  • Zarafeta D., Moschidi D., Ladoukakis E., Gavrilov S., Chrysina E., Chatziioannou A., Kublanov I., Skretas G., Kolisis F.N. Metagenomic mining for thermostable esterolytic enzymes uncovers a new family of bacterial esterases. Sci. Reports. 2016 In press.
  • Sorokin D., Messina E., Smedile F., Roman P., Sinninghe-Damste J., Golyshin P., Ciordia S., Mena M., Ferrer M., Kublanov I., Samarov N., Toshchakov S., La Cono V. and Yakimov M.. Discovery of anaerobic lithoheterotrophic haloarchaea, ubiquitous in hypersaline habitats. ISME J. 2016. In press.
  • Sorokin D., Kublanov I., Gavrilov S., Rojo D., Roman P., Golyshin P., Slepak V., Smedile F., Messina E., La Cono V., Ferrer M. and Yakimov M. Elemental sulfur and acetate can support life of a novel strictly anaerobic haloarchaeon. ISME J. 2016. 10. 240-252. doi:10.1038/ismej.2015.79.
  • Golyshina O., Kublanov I.V., Hai T., Korzhenkov A., Lunsdorf H., Nechitaylo T., Gavrilov S., Toshchakov S., Golyshin P.N. Biology of archaea from a novel family Cuniculiplasmataceae (Thermoplasmata) ubiquitous in hyperacidic environments. Sci. Reports. 2016. 6: 39034. doi:10.1038/srep39034.
  • Sorokin D.Y., Toshchakov S.V., Kolganova T.V., Kublanov I.V. 2015. Halo(natrono)archaea isolated from hypersaline lakes utilize cellulose and chitin as growth substrates. Frontiers Microbiol. 6: 942.
  • Placido A., Hai T. , Ferrer M., Chernikova T.N., Distaso M., Armstrong D., Yakunin A.F., Toshchakov S.V., Yakimov M.M., Kublanov I.V., Golyshina O.V., Pesole G., Ceci L.R., Golyshin P.N. 2015. Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase. Appl Microbiol Biotechnol. 99(23): 10031-10046.
  • Menzel P, Gudbergsdottir SR, Rike AG, Lin L, Zhang Q, Contursi P, Moracci M, Kristjansson JK, Bolduc B, Gavrilov S, Ravin N, Mardanov A, Bonch-Osmolovskaya E, Young M, Krogh A, Peng X. 2015. Comparative Metagenomics of Eight Geographically Remote Terrestrial Hot Springs. Microb Ecol. 70(2):411-24. doi:10.1007/s00248-015-0576-9.
  • Sant’Anna FH, Lebedinsky AV, Sokolova TG, Robb FT, Gonzalez JM. Analysis of three genomes within the thermophilic bacterial species Caldanaerobacter subterraneus with a focus on carbon monoxide dehydrogenase evolution and hydrolase diversity. BMC Genomics. 2015. 16:757.
  • Nesbo CL, Swithers K, Dahle H, Haverkamp THA, Birkeland N-K, Sokolova T, Kublanov I, Zhaxybayeva O. Evidence for extensive gene flow and Thermotoga subpopulations in subsurface and marine environments. ISME J. 2014. V. 9. P. 1532-1542. doi:10.1038/ismej.2014.238.
  • Podosokorskaya O.A., Kadnikov V.V., Gavrilov S.N., Mardanov A.V., Merkel A.Y., Karnachuk O.V., Ravin N.V., Bonch-Osmolovskaya E.A., and Kublanov I.V.. Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae. Environ. Microbiol. 2013. 15. 1759-1771. http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12067/abstract
  • Podosokorskaya O.A., Bonch-Osmolovskaya E.A., Novikov A.A., Kolganova T.V., Kublanov I.V. Ornatilinea apprima gen. nov., sp. nov., a first cellulolytic representative of class Anaerolineae. Int. J. Syst. Evol. Microbiol. 2013. 63: 86-92.
  • Kim Y.J., Lee H.S., Kim E.S., Bae S.S., Lim J.K., Matsumi R., Lebedinsky A.V., Sokolova T.G., Kozhevnikova D.A., Cha S.S., Kim S.J., Kwon K.K., Imanaka T., Atomi H., Bonch-Osmolovskaya E.A., Lee J.H., Kang S.G. Formate-driven growth coupled with H(2) production. Nature. 2010. 467(7313):352-5. doi: 10.1038/nature09375.